Monday, December 18, 2017

Antioxidant and Antimicrobial Properties of Chestnut Honey


Antioxidant and antimicrobial properties of Casteanea sativa Miller chestnut honey produced on Mount Etna (Sicily)

Nat Prod Res. 2017 Dec 13:1-8

The aim of this study was the evaluation of antibacterial and antioxidant properties of Monofloral Etna Castanea sativa Miller honeys. Escherichia coli ATCC 25,922, Pseudomonas aeruginosa ATCC 27,853, Enterococcus faecalis ATCC 29,211 and Staphylococcus aureus ATCC 29,213 were investigated for their susceptibilities to two different honeys. Antioxidant activity was evaluated by ORAC, NO scavenger assays, FRAP and DPPH.

Antioxidant activity and antibacterial properties were compared with chestnut honeys from different geographical areas and with Manuka honey. UPLC-MS/MS was used for major components characterisation.

Sunday, December 17, 2017

Brazilian Stingless Bee Propolis High in Phenols, Flavonoids, Antioxidants

Antioxidant Activity of a Geopropolis from Northeast Brazil: Chemical Characterization and Likely Botanical Origin

Evid Based Complement Alternat Med. 2017;2017:4024721

Geopropolis is a product containing wax, plant resin, and soil particles. It is elaborated by stingless bees of tribe Meliponini. Methanol extracts of sample of geopropolis produced by Scaptotrigona postica ("mandaguari") in the state of Rio Grande do Norte (RN, northeast Brazil) were analyzed for the determination of standard parameters (total phenols, total flavonoids, and radical scavenging activity) and chemical characterization by HPLC-DAD-MS/MS analysis.

The sample analyzed has high contents of total phenols and flavonoids, as well as high antioxidant activity. The constituents characterized were mainly flavonols, such as quercetin methyl ethers, and methoxychalcones. Such chemical profile is similar to the composition of a green propolis from the same area of RN, which is produced by Africanized Apis mellifera, using shoot apices of Mimosa tenuiflora, popularly known as "jurema-preta."

This finding provides evidence that "mandaguari" geopropolis and honeybee propolis have the same botanical origin in RN. The sharing of a plant resin source by phylogenetically distant bees (Apinae and Meliponinae) suggests that bee genetic factors play little role in the choice of plants for resin collection and that the availability of potential botanical sources plays a decisive role.

Saturday, December 16, 2017

Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens

In vitro and in vivo activity of Manuka honey against NDM-1-producing Klebsiella pneumoniae ST11

Future Microbiol. 2018 Jan;13:13-26

AIM:

To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo.

MATERIALS & METHODS:

Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system. Genetic analysis of bla NDM-1 was done by PCR, pulsed-field gel electrophoresis and DNA hybridization. In vitro and in vivo efficacy of Manuka honey was performed by microbroth dilution assay and BALB/c mice model respectively.

RESULTS:

K. pneumoniae ST11 displayed resistance to commonly used antibiotics. bla NDM-1 was located on 150 and 270kb plasmids. Minimum inhibitory concentration and minimum bactericidal concentration of Manuka honey was 30% (v/v) and substantial reduction of bacterial mean log value (>1 log) was observed in mice. Histological analysis of mice liver and kidneys demonstrated mild to moderate inflammation.

CONCLUSION:

Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens.

Friday, December 15, 2017

Bee Venom Used to Treat Arthritis


Bee venom used to treat arthritis in Norwich woman

NORWICH, Kan. (KAKE) - A Norwich, Kansas woman has a unique approach to treating her arthritis by using bee venom as a medication.

Sharon Rowan says she suffers from debilitating arthritis in her hands and one day, she had enough and knew she needed a new treatment.

“It was enough that the joints would just throb. It hurt to write, it hurt to do anything,” Rowan said. “That is when it’s time to think about it, its time to minimize this pain.”

She had friends that used bee venom as a treatment for arthritis and she gave it a try. Rowan has been an avid beekeeper for the past 50 years.

“I put a bee down there on it and it worked, because it quit hurting,” Rowan said of her hand pain from arthritis. “I talked to my doctor about it and he said it works as good as my shot will so go for it.”

Thursday, December 14, 2017

'Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens'

In vitro and in vivo activity of Manuka honey against NDM-1-producing Klebsiella pneumoniae ST11

Future Microbiol. 2017 Dec 11

AIM:

To determine the therapeutic potential of Manuka honey against New Delhi metallo-β-lactamase-1-producing Klebsiella pneumoniae ST11 in vitro and in vivo.

MATERIALS & METHODS:

Carbapenamases and metallo-β-lactamases-producing K. pneumoniae ST11 isolated from blood culture was confirmed by VITEK-2® system, matrix-assisted laser desorption ionization-time of flight and multilocus sequence typing, followed by determination of minimum inhibitory concentration (μg/ml) using VITEK-2 system. Genetic analysis of bla NDM-1 was done by PCR, pulsed-field gel electrophoresis and DNA hybridization. In vitro and in vivo efficacy of Manuka honey was performed by microbroth dilution assay and BALB/c mice model respectively.

RESULTS:

K. pneumoniae ST11 displayed resistance to commonly used antibiotics. bla NDM-1 was located on 150 and 270kb plasmids. Minimum inhibitory concentration and minimum bactericidal concentration of Manuka honey was 30% (v/v) and substantial reduction of bacterial mean log value (>1 log) was observed in mice. Histological analysis of mice liver and kidneys demonstrated mild to moderate inflammation.

CONCLUSION:

Manuka honey can be used as an alternate therapeutic approach for management of New Delhi metallo-β-lactamase-producing pathogens.

Wednesday, December 13, 2017

New Zealand Trademarks Manuka Honey


NZ trademarks mānuka honey and officials create new definition

Many New Zealand beekeepers are celebrating the sweet taste of a landmark decision by United Kingdom authorities to accept the term mānuka honey for trade marking.

The decision reached overnight by the United Kingdom Trade Mark Registry recognising mānuka honey as a certification mark means the New Zealand beekeeping industry can seek further protection of the term.

At the same time Agriculture Minister Damien O'Connor announced the Ministry for Primary Industries had produced a definition for mānuka honey.

He said the decision would not please all producers, but would safeguard the industry from cowboy operators and protect New Zealand's trade reputation.

"The scale of the problem was never truly identified but what we know is there was more manuka honey sold than was being produced, so obviously some cowboy operators were mixing honey. That's not good for our reputation or for customers," O'Connor said...

Tuesday, December 12, 2017

Cytotoxic Effect of Biologically-Transformed Propolis

Biologically Transformed Propolis Exhibits Cytotoxic Effect on A375 Malignant Melanoma Cells In Vitro

Proceedings 2017, 1(10), 1059

Propolis has been used for its health benefits, due to high phenolic content. Recently it has been shown that the extraction methods which yielded phenolic molecules, affected the anti-oxidant and anticancer effect of propolis.

In our previous study we showed that biotransformation of propolis via Lactobacillus plantarum might increase antioxidative effect. In this study, we investigated the cytotoxic effect of this propolis sample on A375 melanoma cells. The propolis samples were extracted in water. The phenolic molecules were determined with LC MS/MS. Cytotoxicity was evaluated by means of the WST.

Water-extracted propolis samples were incubated with L. plantarum (1.5%) in 37 °C for 24 h. A375 cells were treated by propolis with doses of from 25 to 1000 µg/mL, for periods of 24 h, 48 h and 72 h. Cytotoxicity MTT tests were performed. The significantly high phenolic compounds mainly; Quercetine (514 ng/mL), rutin (623 ng/mL), ellagic acid (331 ng/mL), epicatechin (125 ng/mL) were found in propolis samples IC50 values were 412.5 µg/mL (24 h) and 314 µg/mL (48 h) and 353 µg/mL (72 h).

In conclusion, our data showed that the cytotoxic effect of biologically transformed propolis which have high content of rutin, quercetin, ellagic acid, epicatechin. Biotransformation might be a useful strategy to increase bioavailability of phenolic molecules in propolis.

Monday, December 11, 2017

Royal Jelly May May Be a Treatment for Alzheimer’s Disease, Colitis, Crohn’s Disease, Menopausal Symptoms, Diabetes, Osteoporosis


10 Health Benefits of Royal Jelly and Why It May Be A Treatment for Alzheimer’s Disease

By Kaitlin Covel Dec 9 2017

Royal Jelly is not the magic pill cure-all of the natural world, but the incredible health benefits do give it an almost miraculous reputation considering that scientific studies testify to its potential to positively treat a variety of illnesses and symptoms including that of Alzheimer’s Disease.
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What is royal jelly you ask? Royal jelly is the substance milked from the jaws of nurse bees in the hive exclusively for the queen bee. Royal jelly contains significant amounts of protein that are important for cell growth and reproduction as well as carbohydrates, fats, free amino acids, vitamins and minerals. It is interesting to note that royal jelly is rich in B vitamins, the 8 amino acids humans cannot produce, and potassium, magnesium, calcium, copper, sulfur, zinc, iron and manganese as well. No wonder royal jelly is considered a wellspring of nutrients. Here are the top 10 health benefits of royal jelly:

1. Alzheimer’s Disease Treatment

Research conducted recently demonstrated that royal jelly benefits neural function which indicates that it may improve short-term memory for patients with Alzheimer’s disease. The study was published in Advanced Biomedical Research. The powerful antioxidants in Royal Jelly make it an effective and natural treatment for fighting free radical attacks and oxidative stress which occur in many neurodegenerative diseases like Alzheimer’s disease.

2. Immune System Modulator

According to research published in a 2001 study in the journal International Immunopharmacology, royal jelly can boost the functions of the immune system. Histamine responses to allergens were suppressed immediately, evidencing that it may help to relieve allergies of a seasonal nature. Hopefully more research will be completed to validate royal jelly’s usefulness in treating allergies.

3. Colitis, Crohn’s Disease, and IBS Treatment

A European study found that royal jelly protected the mucosa of the colon by limiting the negative effects of the acidic substance that was given to the rats with colitis. Another finding in the study was that royal jelly protected against erosion of the cells in the colon. To replicate the results of this study in humans, the researchers concluded that a 140 pound person would need to take approximately 10 grams of royal jelly per day. In order to get this amount, a freeze-dried dose of royal jelly would be about 2 teaspoons and a fresh dose would be about 2 tablespoons....

Sunday, December 10, 2017

Hydrophilic Bioactive Components of Greek Royal Jelly

Targeted profiling of hydrophilic constituents of royal jelly by hydrophilic interaction liquid chromatography-tandem mass spectrometry

J Chromatogr A. 2017 Nov 13. pii: S0021-9673(17)31667-9

In the present work a Hydrophilic Interaction Liquid Chromatography-tandem Mass Spectrometry (HILIC-MS/MS) method was developed for the efficient separation and quantification of a large number of small polar bioactive molecules in Royal Jelly.

The method was validated and provided satisfactory detection sensitivity for 88 components. Quantification was proven to be precise for 64 components exhibiting good linearity, recoveries R% > 90% for the majority of analytes and intra- and inter-day precision from 0.14 to 20% RSD.

Analysis of 125 fresh royal jelly samples of Greek origin provided useful information on royal jelly's components revealing lysine, ribose, proline, melezitose and glutamic acid to be in high abundance. In addition the occurrence of 18 hydrophilic nutrients which have not been reported previously as royal jelly constituents is shown.

Saturday, December 09, 2017

Indian Propolis Shows Anti-Cancer Effect

Standardization, chemical profiling, in vitro cytotoxic effects, in vivo anti-carcinogenic potential and biosafety profile of Indian propolis

J Ayurveda Integr Med. 2017 Dec 4. pii: S0975-9476(17)30185-7

BACKGROUND:

Propolis from apiculture is known for wide range of medicinal properties owing to its vast chemical constituents including polyphenols, flavonoids and anticancer agent Caffeic acid phenethyl ester (CAPE).

OBJECTIVES:

The objective of the study was to extract and standardize Indian propolis (IP) with respect to selected markers by newly developed High performance liquid chromatography (HPLC) method, to evaluate in vitro and in vivo anticancer activity and biosafety of Indian propolis.

MATERIALS AND METHODS:

IP was extracted, optimized and standardized using a newly developed and validated HPLC method for simultaneous estimation of caffeic acid, apigenin, quercetin and CAPE. The standardised ethanolic extract of IP (EEIP) was screened for in vitro cytotoxicity using sulforhodamine B (SRB) assay, in vivo anti-carcinogenic effect against Dalton's Lymphoma ascites (DLA) cells, hemolytic effect and pesticide analysis.

RESULTS:

The EEIP was found to contain more amount of total flavonoids (23.61+ 0.0452 mg equivalent of quercetin/g), total polyphenolics (34.82 + 0.0785 mg equivalent of gallic acid/g) and all selected markers except caffeic acid compared to all other extracts. EEIP showed better anti-cancer potential than CAPE on MCF-7 and HT-29 cell line and significant (p < 0.01) in vivo anti-carcinogenic effects against DLA in comparison with 5-fluorouracil. EEIP was found to be non-hemolytic.

CONCLUSION:

From in vitro cytotoxicity, in vivo anti-carcinogenicity and biosafety studies it can be concluded that the standardized EEIP is safe and can be considered for further development as a biomedicine.

Friday, December 08, 2017

Manuka Honey Helps Treat Severe Atopic Eczematous Dermatitis

Manuka Honey: A Case Study of Severe Atopic Eczematous Dermatitis Reaction to Henna Tattoo

Plast Surg Nurs. 2017 Oct/Dec;37(4):154-157

Many mainstream medications were derived from plants and originally utilized in patient management well prior to the extensive research and testing processes of current pharmaceutical standards. The evolution of therapeutic management within the pharmaceutical and skin care industry often uses synthetic processing of products with less of a focus on the natural ingredients from which they were originally derived.

However, more recently there has been a shift in pharmacological management to include the therapeutic use of more holistic medicines and practices and thus a broadening of the uses of nontraditional medical treatment options. This has been seen in the use of treatments, such as Manuka honey, for skin conditions and dermal injuries. It is often with off-label uses, or conditions resistant to other treatments, that then prompt the use of holistic products and the true value of the product is validated.

As with the following case study, the example of the use of Manuka honey on a severe atopic contact dermatitis eczematous reaction provides further documentation and supportive evidence of the potential efficacy of the properties of this particular natural product.

Thursday, December 07, 2017

Propolis Component Useful in Treatment of Acute Lung Injury

Discovery of caffeic acid phenethyl ester derivatives as novel myeloid differentiation protein 2 inhibitors for treatment of acute lung injury

Eur J Med Chem. 2017 Dec 1;143:361-375

Myeloid differentiation protein 2 (MD2) is an essential molecule which recognizes lipopolysaccharide (LPS), leading to initiation of inflammation through the activation of Toll-like receptor 4 (TLR4) signaling. Caffeic acid phenethyl ester (CAPE) from propolis of honeybee hives could interfere interactions between LPS and the TLR4/MD2 complex, and thereby has promising anti-inflammatory properties.

In this study, we designed and synthesized 48 CAPE derivatives and evaluated their anti-inflammatory activities in mouse primary peritoneal macrophages (MPMs) activated by LPS. The most active compound, 10s, was found to bind with MD2 with high affinity, which prevented formation of the LPS/MD2/TLR4 complex. The binding mode of 10s revealed that the major interactions with MD2 were established via two key hydrogen bonds and hydrophobic interactions. Furthermore, 10s showed remarkable protective effects against LPS-caused ALI (acute lung injury) in vivo.

Taken together, this work provides new lead structures and candidates as MD2 inhibitors for the development of anti-inflammatory drugs.

Wednesday, December 06, 2017

Honey Effective as Treatment for Chemotherapy-Induced Mucositis in Paediatric Oncology Patients

Global Health Journal Club: Is Honey Effective as a Treatment for Chemotherapy-induced Mucositis in Paediatric Oncology Patients?

J Trop Pediatr. 2017 Nov 30

Oral mucositis (OM) is an inflammatory response of mucosal epithelium to the cytotoxic effects of chemotherapy and radiotherapy causing severe oral pain and ulceration, which may complicate the management of cancer. The Mucositis Prevention Guideline Development Group has developed an international guideline for the prevention of mucositis in children receiving treatment for cancer or undergoing haematopoietic stem cell transplantation.

Evidence-based preventative strategies include cryotherapy, low-level light therapy and keratinocyte growth factor. However, these strategies are often not available in resource-poor settings. There is some evidence that honey may be a suitable treatment for OM in adult patients. We performed a literature search of 11 databases to find papers exploring the use of honey to treat chemotherapy-associated mucositis in paediatric oncology patients.

We found four papers, which provide Grade C evidence that honey is effective as a preventative and therapeutic measure for OM in paediatric oncology patients.

Monday, December 04, 2017

Give Children Honey and Lemon, Not Cough Medicine Says Top Doc

By Henry Bodkin, 1 December 2017 

Children should not be given cough medicine but should instead be treated with “old fashioned” honey and lemon, a leading paediatrician has said.

Using over-the-counter syrups and medications risk unintentionally overdosing toddlers and causing “toxic” events, according to Dr Oliver Bevington, from the Royal College of Paediatrics and Child Health.

Chair of the college’s trainees’ committee, Dr Bovington said there was no evidence cough medicines work and added they can end “actually end up doing more harm than good”.

Most childhood coughs and colds get better simply with rest, fluids and possibly paracetamol or ibuprofen, he said.

The intervention follows an announcement by the NHS earlier this year saying it would no longer fund free cough mixture.

“A lot of cough and cold medicines contain active ingredients such as nasal decongestants, antihistamines and cough suppressors that may, in large doses, have adverse effects or be toxic if consumed in large quantities, particularly to the under-sixes who are much more susceptible,” he said.

“The bottom line is there is absolutely no evidence that cough medicines work as there has been very little research with regards to their use and, potentially, they could actually do children more harm than good.

“My advice for parents would be to stick to old fashioned honey and lemon.”...

Sunday, December 03, 2017

Bee Venom Component Inhibits Tumor Growth

The anti-hepatocellular carcinoma activity of Mel-P15 is mediated by natural killer cells

Oncol Lett. 2017 Dec;14(6):6901-6906

Mel-P15 is a peptide derived from melittin, the main toxic component in the venom of the European honeybee Apis mellifera.

In the present study, the antitumor effects of Mel-P15 and the underlying molecular mechanisms of these effects in vivo were investigated. Mel-P15 directly stimulated natural killer (NK) cell cytotoxicity in vitro, which was increased to 55.45% at a 4 µg/ml dose of Mel-P15. In the mouse liver cancer (H22) xenograft mice model, Mel-P15 suppressed tumor growth in vivo; the tumor inhibitory rate was 61.15% following treatment with 2 mg/kg Mel-P15. In addition, the immune response was activated following Mel-P15 treatment. Mel-P15 treatment increased the spleen and thymus indices, promoted splenocyte proliferation, stimulated NK cytotoxicity and upregulated the secretion of cytokines, including interleukin-2, interferon-γ and tumor necrosis factor-α. In addition, the tumor inhibitory effect of Mel-P15 on BEL-7402-bearing nude mice was abrogated by the selective depletion of NK cells via the intraperitoneal injection of an anti-asialo GM-1 antibody.

The results suggest that Mel-P15 inhibits tumor growth in vivo by promoting NK cell cytotoxicity. Mel-P15 may therefore be a potential immunotherapy candidate for the treatment of hepatocellular carcinoma.